Tartrate-resistant Acid Phosphatase-positive Gaucher Cells

Expression of tartrate-resistant acid phosphatase.

Enzyme immunoassay for tartrate-resistant acid phosphatase.

An immunochemical method for quantitative analysis of the tartrate-resistant acid phosphatase (EC 3.1.3.2), band 5, is presented. This method involves precipitation of the enzyme from the serum by the antibody specific to band 5 and by sheep anti-rabbit immunoglobulin, followed by analysis of the enzyme activity in the precipitate. The precipitation procedure eliminates the interferences of the...

A tartrate-resistant acid phosphatase from Gaucher spleen. Purification and properties.

”he acid phosphatase activity that is elevated 3to 5fold in the spleen in Gaucher’s disease can be resolved into two principal isoenzymes by chromatography on sulphopropyl-Sephadex: one tartrate-sensitive component does not bind to the resin whereas the second acid phosphatase, which is tartrate-resistant, binds to the column and can be eluted by a buffer containing 0.4 M sodium chloride. We ha...

Generation of CD1+RelB+ dendritic cells and tartrate-resistant acid phosphatase-positive osteoclast-like multinucleated giant cells from human monocytes.

We previously showed that granulocyte-macrophage colony-stimulating factor (GM-CSF) and macrophage colony-stimulating factor (M-CSF) stimulate the differentiation of human monocytes into two phenotypically distinct types of macrophages. However, in vivo, not only CSF but also many other cytokines are produced under various conditions. Those cytokines may modulate the differentiation of monocyte...

Structure, function, and regulation of tartrate-resistant acid phosphatase.

The tartrate-resistant acid phosphatases (TRAPs) are a class of metalloenzymes that catalyze the hydrolysis of various phosphate esters and anhydrides under acidic reaction conditions. Because the bound metal ions confer an intense color on these enzymes they are also known as purple acid phosphatases (PAPs). Resistance to inhibition by high concentrations of the competitive inhibitor L 1 tartr...